What does the line look like?
Arduino prints Serial.print(temperature); Serial.print(","); Serial.println(humidity); with temperature = 22.4 and humidity = 48. Write the exact characters that travel over USB, including the invisible ones.
println adds a newline (\n). There is no space after the comma.22.40,48.00 followed by \n. (Arduino may print extra zeros — the important part is number,comma,number,newline.)